![]() Further association mapping using existing data to identify genetic variants associated with stop codon readthrough is likely to reveal mechanistic insights on regulation of this process. Proposed project: The goal of the current project is to adapt the existing methodology to identify translation readthrough events and to further evaluate potential biological importance of these events. Using the fine structure pattern in ribosome profiling data, we have developed a method to identify coding regions that are translated transcriptome-wide (Raj et al. This discovery highlighted potential biological roles of stop codon readthrough translation. Ribosome profiling-based approaches have revolutionized our ability to monitor every step of protein synthesis in vivo, allowing one to measure the rate of protein synthesis across the proteome, annotate the protein coding capacity of genomes, monitor localized protein synthesis, and explore cotranslational folding and targeting. More importantly, a stop codon readthrough isoform of VEGF is recently discovered and found to play an antagonist role in angiogenesis (Eswarappa et al. The manuscript would be strengthened by including a short paragraph in the Introduction that informs the reader of currently available resources, e.g. Recently, the advance of genome technology has started generating results pointing to evidence of programed stop codon readthrough of unknown biological significance (Dunn et al. Ribosomal profiling is an emerging experimental technology to measure protein synthesis by sequencing short mRNA fragments undergoing translation in ribosomes. Stop codon readthrough has been considered as rare mistakes. One key rule of protein translation is that the translation elongation complex exits the transcript when it reaches the first in frame stop codon. we refer the reader to S1 Fig 14, 15, 18, 2549. While a basic bioinformatic understanding is becoming more. Project title: Statistical approaches for analysis of ribosome profiling data: regulation of stop codon readthrough during protein translationīackground: Protein translation is an intricate process precisely controlled to ensure accurate protein production. cess ribosome profiling data by acting as a reference implementation of current best practices for ribosome profiling analysis. ![]() Location: Melbourne Integrative Genomics, University of Melbourne You must be a member to see who’s a part of this organization. Ribosome Profiling has 11 repositories available. #Ribosome profiling viewer softwareA software ecosystem for ribosome profiling. Statistical approaches for analysis of ribosome profiling data: regulation of stop codon readthrough during protein translationĪvailable for: MSc and undergraduate research projects. Ribosome Profiling has 11 repositories available. ![]()
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